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The motion and deformation of a neutrally buoyant drop in a rectangular channel experiencing a pressure-driven flow at a low Reynolds number has been investigated both experimentally and numerically. A moving-frame boundary-integral algorithm was used to simulate the drop dynamics, with a focus on steady-state drop velocity and deformation. Results are presented for drops of varying undeformed diameters relative to channel height ($$D/H$$), drop-to-bulk viscosity ratio ($$\lambda$$), capillary number ($$Ca$$, ratio of deforming viscous forces to shape-preserving interfacial tension) and initial position in the channel in a parameter space larger than considered previously. The general trend shows that the drop steady-state velocity decreases with increasing drop diameter and viscosity ratio but increases with increasing$$Ca$$. An opposite trend is seen for drops with small viscosity ratio, however, where the steady-state velocity increases with increasing$$D/H$$and can exceed the maximum background flow velocity. Experimental results verify theoretical predictions. A deformable drop with a size comparable to the channel height when placed off centre migrates towards the centreline and attains a steady state there. In general, a drop with a low viscosity ratio and high capillary number experiences faster cross-stream migration. With increasing aspect ratio, there is a competition between the effect of reduced wall interactions and lower maximum channel centreline velocity at fixed average velocity, with the former helping drops attain higher steady-state velocities at low aspect ratios, but the latter takes over at aspect ratios above approximately 1.5.